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rabbit anti-zo-2 (1:500 wb)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rabbit anti-zo-2 (1:500 wb)
    Rabbit Anti Zo 2 (1:500 Wb), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+zo+2/pm39882731-328-23-34
    Average 90 stars, based on 1 article reviews
    rabbit anti-zo-2 (1:500 wb) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Apical surface supracellular mechanical properties in polarized epithelium using noninvasive acoustic force spectroscopy
    Article Snippet: .. Gels were transferred to nitrocellulose filters and then blocked in a solution of PBS (Life Technologies) and 10% non-fat dry milk powder (NFDM) for 1 h. Filters were incubated for 1 h in primary antibodies rat ZO-1 monoclonal (1:500; homemade ), Ms E-cadherin (24E10; 1:1000; Cell Signaling Technologies), rabbit non-muscle myosin 2B (909901; 1:1,000; BioLegend, San Diego, CA), and rabbit ZO-2 (38-9100; 1:1000; ThermoFisher Scientific) diluted in a solution of PBS, 5% NFDM, and 1% Tween-20 (PBS-T); washed four times for 5 min each in PBS-T; and incubated for 30 min with 1:2,000 dilution of the appropriate species-specific secondary antibodies anti-rat 680 (612-144-120), anti-mouse 680 (610-730-124), and anti-rabbit 800CW (611-731-127) (Rockland, Gilbertsville, MD). .. After four more washes in PBS-T, filters were imaged with the Odyssey infrared imaging system (Licor Biosciences, Lincoln, NE).

    Article Title: Apical surface supracellular mechanical properties in polarized epithelium using noninvasive acoustic force spectroscopy.
    Article Snippet: .. Gels were transferred to nitrocellulose filters and then blocked in a solution of PBS (Life Technologies) and 10% non-fat dry milk powder (NFDM) for 1 h. Filters were incubated for 1 h in primary antibodies rat ZO-1 monoclonal (1:500; homemade42), Ms E-cadherin (24E10; 1:1000; Cell Signaling Technologies), rabbit non-muscle myosin 2B (909901; 1:1,000; BioLegend, San Diego, CA), and rabbit ZO-2 (38-9100; 1:1000; ThermoFisher Scientific) diluted in a solution of PBS, 5% NFDM, and 1% Tween20 (PBS-T); washed four times for 5 min each in PBS-T; and incubated for 30 min with 1:2,000 dilution of the appropriate species-specific secondary antibodies antirat 680 (612-144-120), anti-mouse 680 (610-730-124), and anti-rabbit 800CW (611-731-127) (Rockland, Gilbertsville, MD). .. After four more washes in PBS-T, filters were imaged with the Odyssey infrared imaging system (Licor Biosciences, Lincoln, NE).



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